Human IL-10 ELISA Kit (96-well)

CAT#: EA100036

Human IL-10 ELISA Kit (96-well)

Size: 1 x 48 wells 1 x 96 wells


USD 415.00

3 Weeks*

Size
    • 1 x 96 wells

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Other products for "IL10"

Specifications

Product Data
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hours
Signal Colorimetric
Curve Range 15.625-1000pg/ml
Sample Type Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume 20 uL
Specificity Natural and recombinant Human IL-10 Ligand
Sensitivity 7pg/mL
Reactivities Human
Interference No significant interference observed with available related molecules.
Components
  • Aluminium pouches with a Microwell Plate coated with antibody to human IL-10 (8x12)
  • 1 vials human IL-10 Standard lyophilized, 1000 pg/ml upon reconstitution
  • 1 vials concentrated Biotin-Conjugate anti-human IL-10 antibody
  • 1 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 2 pieces Adhesive Films
  • package insert
Background

Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (TH2) clones that suppressed the production of cytokines by T helper 1 (TH1) clones responding to antigen stimulation in the presence of monocyte/macrophage antigen-presenting cells .

The human homologue of mouse IL-10 was subsequently cloned by cross-hybridization . Human and mouse IL-10 are 81% and 73% identical at the nucleotide and amino acid levels, respectively. Mouse, rat and human IL-10 cDNAs encode 178 amino acid (aa) residue precursor polypeptides with hydrophobic signal peptides that are cleaved to generate 160 aa residue mature proteins. There is at least 73% aa identity between the proteins from the three species. In contrast to mouse IL-10, human IL-10 appears to be non-glycosylated. Both mouse and human IL-10 exist as non-covalently-linked homodimers in solution. Although human IL-10 is active on mouse cells, mouse IL-10 does not show species cross-reactivity on human cells.

IL-10 is a pleiotropic cytokine that can exert either immunosuppressive or immunostimulatory effects on a variety of cell types. human IL-10 can inhibit monocyte/macrophage-dependent, antigen-stimulated cytokine synthesis by human PBMNC and NK cells . L-10 can inhibit production or function of a macrophage membrane-bound costimulator required for activation of T cells and NK cells.

IL-10 is a potent modulator of monocyte/macrophage function. IL-10 also enhances the release of soluble TNF receptors and inhibits the expression of surface ICAM-1 and B7 . Finally, IL-10 has been reported to suppress the synthesis of superoxide anion plus reactive oxygen intermediates (ROI), and either inhibit or facilitate nitric oxide synthesis, depending on the time of exposure to activated macrophages . IL-10 has marked effects on B cells. IL-10 also has documented activity on endothelial cells, where it mimics IL-4 , and on thymocytes and mast cells, where it acts as a growth costimulator .

Gene Symbol IL10
Standard Curve
Representative standard curve for IL-10 ELISA. IL-10 was diluted in serial two-fold steps in Sample Diluent.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.