Human Resistin ELISA Kit (96-well)

CAT#: EA100064

Human Resistin ELISA Kit (96-well)

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 7.8-500pg/ml
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Human Resistin Ligand
• Sensitivity: 4pg/mL
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with antibody to human Resistin (8X12)
  • 2 vials human Resistin Standard lyophilized, 1000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human Resistin antibody
  • 2 vials Streptavidin-HRP solution
  • 4 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
• Background:

  Resistin, also known as Found In Inflammatory Zone 3 (FIZZ3) or Adipocyte Secreted Factor(ADSF), is a member of a protein family known as the Resistin-like molecules (RELMs). It is perhaps best known for its potential as a link between obesity and the development of insulin resistance . Other members of this family include RELM-./FIZZ1 and RELM-., which are described in rodents but as yet have no identified human counterparts, and RELM-./FIZZ2. The Resistin amino acid (aa) sequence contains a putative N-terminal signal sequence and a motif containing 11 cysteine residues, 10 of which are characteristic of the RELM family. The protein is thought to be secreted as a dimer and potentially exists in higher order molecular structures resulting from interactions between Resistin dimers or other members of the RELM family . A splice variant in the rat, lacking the signal sequence and localized predominantly to the nucleus, has also been described . A large 3' intron is the primary reason that the mouse genomic sequence is 3-fold larger than the corresponding human sequence . Mouse and human Resistin share only 53 percent identity at the aa level and exhibit differences in expression patterns . In mouse, expression appears primarily inadipose tissues . Although some human studies suggest Resistin is expressed by adipose tissues as well, the most significant source appears to be blood mononuclear cells . In humans, Resistin is also reported to be expressed by pre-adipocytes , placenta , pancreatic islets , and primary leukemia cells . A receptor for Resistin has not yet been described.

  Resistin acquired initial attention as a potential link between obesity and glucose regulation. Serum levels were shown to increase in diet-induced and genetic forms of obesity in mice (ob/ob and db/db) and decrease in response to insulin sensitizing drugs (TZDs) . In addition, function-blocking Resistin antibodies enhanced insulin actions while treatment with recombinant Resistin caused glucose intolerance and insulin resistance . Resistin knockout mice exhibit decreased fasting blood glucose levels as a result of reduced hepatic output . To establish a physiological role in humans, several studies have examined whether altered circulating Resistin levels are associated with type 2 diabetes, insulin resistance, and/or obesity. Although some demonstrate significant correlations , others report no correlation , suggesting that in humans fundamental questions remain regarding ResistinÂ?s role in these pathophysiological processes . Resistin expression by human mononuclear cells could indicate a potential role in inflammation. In vitro, Resistin expression by these cells is enhanced by treatment with several pro-inflammatory cytokines including IL-1., TNF-., IFN-., or IL-6 . In addition, Resistin has been shown to activate endothelial cells in vitro, leading to the production of adhesion molecules, Endothelin-1, and chemokines .

• Gene Symbol: RETN
• Standard Curve:
  
  Representative standard curve for Resistin ELISA. Resistin was diluted in serial two-fold steps in Sample Diluent.