Human VEGF ELISA Kit (96-well)

CAT#: EA100066

Human VEGF ELISA Kit (96-well)

Product Images

Representative standard curve for VEGF ELISA. VEGF was diluted in serial two-fold steps in Sample Diluent.

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 31.25-2000pg/ml
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Human VEGF Ligand
• Sensitivity: 7pg/mL
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with antibody to human VEGF (8x12)
  • 1 vials human VEGF Standard lyophilized, 2000 pg/ml upon reconstitution
  • 1 vials concentrated Biotin-Conjugate anti-human VEGF antibody
  • 1 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 2 pieces Adhesive Films
  • package insert
• Background:

  Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is well known for its important roles in regulating both physiological and pathological blood vessel growth . It is a member of the VEGF family that also includes VEGF-B, -C, -D, -E, and PlGF (placental growth factor) . Family members exhibit a cysteine knot motif formed by eight characteristically spaced cysteine residues .

  Various cells and tissues express VEGF including skeletal and cardiac muscle, hepatocytes, osteoblasts, neutrophils, macrophages, keratinocytes, platelets, brown adipose tissue, CD34+ cells, astrocytes, neurons, and endothelial cells . VEGF can be detected in both human plasma and serum samples, with higher levels found in serum due to its release from platelets . VEGF transcription is potentiated in response to hypoxia, oncogenic transformation, and growth factors.

  VEGF has several physiological roles both during development and in the adult. It has a well-documented and critical role in embryonic vasculogenesis . Targeted deletion of a single VEGF allele results in significant defects in vascular development and is embryonic lethal . VEGF acts as an endothelial cell survival factor, mitogen, and inducer of migration, functions that potentially involve an array of signaling molecules including G proteins, PI3 kinase, MAP kinases, FAK, PKC, and NO . VEGF affects bone formation by regulating blood vessel growth and cartilage remodeling, activities important for growth plate morphogenesis . In the adult, VEGF has potential roles in cyclic angiogenesis in the female reproductive system. Other diverse functions include the regulation of hematopoietic development and roles as a neurotrophic factor.

  VEGF has received much attention for its involvement in tumor-associated angiogenesis and its potential as a target for cancer therapy. Numerous studies have demonstrated that VEGF or its receptors are upregulated in several forms of human cancer . Neutralizing VEGF antibodies are shown to reduce tumor growth in cancer models.

• Gene Symbol: VEGFA
• Standard Curve:
  
  Representative standard curve for VEGF ELISA. VEGF was diluted in serial two-fold steps in Sample Diluent.