Human sICAM-1 ELISA Kit (96-well)

CAT#: EA100070

Human sICAM-1 ELISA Kit (96-well)

Size: 1 x 48 wells 1 x 96 wells


USD 415.00

3 Weeks*

Size
    • 1 x 96 wells

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Other products for "ICAM1"

Specifications

Product Data
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hours
Signal Colorimetric
Curve Range 62.5-4000pg/ml
Sample Type Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume 20 uL
Specificity Natural and recombinant Human sICAM-1 Ligand
Sensitivity 31pg/mL
Reactivities Human
Interference No significant interference observed with available related molecules.
Components
  • Aluminium pouches with a Microwell Plate coated with antibody to human sICAM-1 (8?12)
  • 2 vials human sICAM-1 Standard lyophilized, 4000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human sICAM-1 antibody
  • 2 vials Streptavidin-HRP solution
  • 4 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
Background

Intercellular Adhesion Molecule 1 (ICAM-1), also known as CD54, is a nearly ubiquitous transmembrane glycoprotein that plays a key role in leukocyte migration and activation . Human ICAM-1 contains five Ig-like domains in its extracellular domain (ECD) and associates into non-covalently linked dimers . Soluble forms of monomeric and dimeric ICAM-1 (sICAM-1) can be generated via proteolytic cleavage by cathepsin G, elastase, MMP-9, MMP-14/MT1-MMP, and TACE/ADAM17 . In the mouse, alternate splicing generates isoforms that lack particular Ig-like domains and are differentially sensitive to proteolysis . Within the ECD, human ICAM-1 shares 53% amino acid sequence identity with mouse and rat ICAM-1.

The principal binding partners of ICAM-1 are the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) . The multivalency of dimeric ICAM-1 increases its strength of interaction with LFA-1 . ICAM-1 also binds several non-integrin ligands including CD43/sialophorin, fibrinogen, hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes . At sites of inflammation, ICAM-1 is upregulated on endothelial and epithelial cells where it mediates the adhesion and paracellular migration of leukocytes expressing activated LFA-1 and Mac-1 . ICAM-1 ligation prolongs antigen presentation by dendritic cells and promotes T cell proliferation and cytokine release . ICAM-1 activation also participates in angiogenesis, wound healing, and bone metabolism .

Soluble ICAM-1 has been reported in serum, cerebrospinal fluid, urine, and bronchoalveolar lavage fluid . Elevated levels of sICAM-1 in these fluids are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, abdominal fat mass, hypertension, liver disease, and certain malignancies . sICAM-1 promotes angiogenesis and serves as an indicator of vascular endothelial cell activation or damage . It also functions as an inhibitor of transmembrane ICAM-1 mediated activities such as monocyte adhesion to activated endothelial cells and sensitivity of tumor cells to NK cell-mediated lysis .

Gene Symbol ICAM1
Standard Curve
Representative standard curve for sICAM-1 ELISA. sICAM-1 was diluted in serial two-fold steps in Sample Diluent.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.