Human Leptin ELISA Kit (96-well)

CAT#: EA100072

Human Leptin ELISA Kit (96-well)

Product Images

Representative standard curve for Leptin ELISA. Leptin was diluted in serial two-fold steps in Sample Diluent.

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 31.25-2000pg/ml
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinantHuman Leptin Ligand
• Sensitivity: 7pg/mL
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with antibody to human Leptin (8x12)
  • 2 vials human Leptin Standard lyophilized, 2000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human Leptin antibody
  • 2 vials Streptavidin-HRP solution
  • 4 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer
  • Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
• Background:

  Human Leptin (gene name OB) is a 16 kDa, 146 amino acid (aa) residue, non-glycosylated polypeptide that regulates adipose tissue mass and energy balance . Mature human Leptin shares 87% and 84% aa identity with mouse and rat Leptin, respectively. Human Leptin is active in both the mouse and rat systems . Leptin is expressed almost exclusively by adipocytes and its production is influenced by hormones, cytokines and nutrients and circulates in the plasma, crosses the blood-brain barrier, and is present in human breast milk . The human Leptin receptor (designated ObR or LEPR) is a 150 kDa, 1144 aa residue, type I transmembrane glycoprotein of the IL-6 receptor family of Class I cytokine receptors . The gene for ObR undergoes considerable splicing, forming variants a-d with cytoplasmic domains of variable length, plus the potentially soluble form ObRe . The long form, ObRb (formerly OB RL), is expressed mainly in the hypothalamic arcuate nucleus and is essential for signal transduction . In a concentration-dependent manner, Leptin signaling can have diverse effects, causing neurons that express pro-opiomelanocortin (POMC) peptides to reduce food intake, and neurons that express neuropeptide Y and agouti-related protein (NpY and AgRP) to increase food intake . Leptin is fundamentally a Â?starvation signalÂ? that, when low, prompts increased appetite and decreased energy expenditure . Leptin deficiency influences the immune system, depressing Th1 responses and causing increased frequency of infections . Leptin also regulates puberty, blocking the onset of puberty, or of menses if Leptin deficiency exists due to excessive thinness, such as results from starvation, extreme exercise-induced weight loss, anorexia or cancer-induced cachexia .

• Gene Symbol: IL12A
• Standard Curve:
  
  Representative standard curve for Leptin ELISA. Leptin was diluted in serial two-fold steps in Sample Diluent.