Human IL-15 ELISA Kit (48-well)
USD 290.00
3 Weeks*
Specifications
Product Data | |
Format | 48-well strip plate |
Assay Type | Solid Phase Sandwich ELISA |
Assay Length | 3 hours |
Signal | Colorimetric |
Curve Range | 7.8-500pg/ml |
Sample Type | Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) |
Sample Volume | 20 uL |
Specificity | Natural and recombinant Human IL-15 Ligand |
Sensitivity | 4pg/mL |
Reactivities | Human |
Interference | No significant interference observed with available related molecules. |
Components |
|
Background | Interleukin 15 (IL-15) is a 14 kDa cytokine that is structurally and functionally related to IL- 2. Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly . IL-15 associates with IL-15 R. in the endoplasmic reticulum, and this complex is expressed on the cell surface . The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R. are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R./.c on adjacent cells . This enables cells to respond to IL-15 even if they do not express IL-15 R. . Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis . It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells . IL-15 also exerts pleiotropic effects on other hematopoietic cells and nonimmune cells . Ligation of membraneassociated IL-15/IL-15R. complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15R. expressing cells . |
Gene Symbol | IL15 |
Standard Curve |
![]() Representative standard curve for IL-15 ELISA. IL-15 was diluted in serial two-fold steps in Sample Diluent.
|
Documents
{0} Product Review(s)
Be the first one to submit a review