Human IL-15 ELISA Kit (48-well)

CAT#: EA100075

Human IL-15 ELISA Kit (48-well)

Product Images

Representative standard curve for IL-15 ELISA. IL-15 was diluted in serial two-fold steps in Sample Diluent.

Specifications

Product Data

• Format: 48-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 7.8-500pg/ml
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Human IL-15 Ligand
• Sensitivity: 4pg/mL
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with antibody to human IL-15 (8x6)
  • 2 vials human IL-15 Standard lyophilized, 1000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human IL-15 antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
• Background:

  Interleukin 15 (IL-15) is a 14 kDa cytokine that is structurally and functionally related to IL- 2. Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly . IL-15 associates with IL-15 R. in the endoplasmic reticulum, and this complex is expressed on the cell surface . The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R. are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R./.c on adjacent cells . This enables cells to respond to IL-15 even if they do not express IL-15 R. . Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis . It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells . IL-15 also exerts pleiotropic effects on other hematopoietic cells and nonimmune cells . Ligation of membraneassociated IL-15/IL-15R. complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15R. expressing cells .

• Gene Symbol: IL15
• Standard Curve:
  
  Representative standard curve for IL-15 ELISA. IL-15 was diluted in serial two-fold steps in Sample Diluent.