Human IL-32a ELISA Kit (96-well)

CAT#: EA100082

Human IL-32a ELISA Kit (96-well)

Product Images

Representative standard curve for IL-32a ELISA. IL-32a was diluted in serial two-fold steps in Sample Diluent.

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 15.625-1000pg/ml
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Human IL-32a Ligand
• Sensitivity: 7pg/mL
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-32a(8x12)
  • 2 vials human IL-32a Standard lyophilized, 500 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human IL-32a monoclonal antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 3 pieces Adhesive Films
  • package insert
• Background:

  IL-32. is the shortest and most abundant of four potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4) with a predicted unmodified size of 15 kDa. Potential modifications include myristoylation and N-glycosylation. The IL-32. shows increased potency at inducing CXCL2/MIP-2 and CXCL8 expression in PBMC relative to uncleaved IL-32.. Transfected IL-32. was more likely to be cellassociated as compared to IL-32., suggesting an intracellular function. IL-32 is induced by mitogens in peripheral lymphocytes, by IFN. in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn induces cytokine expression.

• Gene Symbol: IL32
• Standard Curve:
  
  Representative standard curve for IL-32a ELISA. IL-32a was diluted in serial two-fold steps in Sample Diluent.