Human IFN-a ELISA Kit (96-well)

CAT#: EA100088

Human IFN-a ELISA Kit (96-well)

Product Images

Representative standard curve for IFN-a ELISA. IFN-a was diluted in serial two-fold steps in Sample Diluent.

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 15.625-1000pg/ml
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Human IFN-a Ligand
• Sensitivity: 7pg/mL
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with antibody to human IFN-a(8X12)
  • 2 vials human IFN-a Standard
  • lyophilized, 500pg/ml upon reconstitution
  • 2 vials HRP-Conjugate solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
• Background:

  IFN-./. R2, also known as IFNAR2, is a 100 kDa glycoprotein in the class II cytokine receptor family. These proteins form heterodimeric receptor complexes that transduce signals from the interferon, IL 10, and IL28 families of cytokines . IFN-./. R2, in association with IFN-./. R1, is required for mediating the antiviral,antiproliferative, and apoptotic effects of the type I interferons IFN-. and IFN-..

  IFN-./. R2 is the principal ligand binding subunit of the receptor. Ligand binding is stabilized by the subsequent association with IFN-./. R1, resulting in the formation of a signaling ternary receptor complex . Mature human IFN-./. R2 consists of a 217 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, a 21 aa transmembrane segment, and a 251 aa cytoplasmic domain. Alternate splicing generates a secreted isoform that corresponds to the ECD and a 50 kDa transmembrane isoform with a substituted and truncated cytoplasmic region . The short isoform is impaired in its ability to activate signaling molecules and functions as a dominant negative receptor subunit . IFN-./. R2 is also subject to presenilin dependent intramembrane proteolysis, resulting in the liberation of nearly the entire ECD as well as the cytoplasmic domain which migrates to the nucleus and can inhibit gene transcription . High concentrations of soluble IFN-./. R2 bind and neutralize IFN-. and IFN-., while lower concentrations prolong the antiviral activity of circulating IFN-. but not IFN-. . Human but not mouse IFN-./. R2 constitutively associates with STAT4, which may account for species specific differences observed in type I interferon responses . Within the ECD, human IFN-./. R2 shares 63%, 60%, and 48% aa sequence identity with bovine, mouse, and ovine IFN-./. R2, respectively.

• Gene Symbol: IFNA1
• Standard Curve:
  
  Representative standard curve for IFN-a ELISA. IFN-a was diluted in serial two-fold steps in Sample Diluent.