Human IL-24/MDA-7 ELISA Kit (96-well)

CAT#: EA100090

Human IL-24/MDA-7 ELISA Kit (96-well)

Size: 1 x 48 wells 1 x 96 wells


USD 415.00

3 Weeks*

Size
    • 1 x 96 wells

Product Images

Other products for "IL24"

Specifications

Product Data
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hours
Signal Colorimetric
Curve Range 62.5-4000pg/ml
Sample Type Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume 20 uL
Specificity Natural and recombinant Human IL-24/MDA-7 Ligand
Sensitivity 30pg/mL
Reactivities Human
Interference No significant interference observed with available related molecules.
Components
  • Aluminium pouches with a Microwell Plate coated with antibody to human IL-24 (8X12)
  • 2 vials human IL-24 Standard lyophilized, 2000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human IL-24 antibody
  • 2 vials Streptavidin-HRP solution
  • 4 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
Background

Interleukin 24(IL-24),also known as mda7 (melanoma differentiation associated gene7),is a member of the IL10 family of helical cytokines. The IL-24 gene encodes a precursor protein of 207 amino acids (aa) that contains a 48 aa signal sequence and an 18 kDa, 158 aa mature segment. There are three potential Nlinked glycosylation sites, at least one of which is used. When secreted, IL-24 is a 3540 kDa phosphorylated glycoprotein that apparently can exist as either a monomer or dimer. It is suggested that glycosylation is essential for activity. Mature human IL-24 shares 69% aa sequence identity with mouse and rat IL-24. Human IL-24 is also active in rodent systems. Cells known to express IL-24 include B cells, CD4+ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. Functionally, IL-24 has diverse activities. At low concentrations on monocytes, it induces type I proinflammatory cytokines such as IFN-., IL-1.,IL-12, and TNF-.. At high concentrations, it is a strong inducer of apoptosis in tumor cells, but not normal cells. IL-24 also has antiangiogenic properties. It directly binds IL-24 receptors on endothelial cells, activating STAT3 and blocking their differentiation. IL-24 binds and signals through two heterodimeric receptor complexes. One complex is the combination of IL-20R. and IL-20R., which is shared with IL-19 and IL-20. The second complex is a combination of IL-22R and IL-20R., which is shared with IL-20.

Gene Symbol IL24
Standard Curve
Representative standard curve for IL-24 ELISA. IL-24 was diluted in serial two-fold steps in Sample Diluent.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.