Human PBEF/ Visfatin ELISA Kit (48-well)

CAT#: EA100091

Human PBEF/ Visfatin ELISA Kit (48-well)

Specifications

Product Data

• Format: 48-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 1.56-100pg/ml
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Human PBEF/ Visfatin Ligand
• Sensitivity: 0.70ng/mL
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with antibody to human PBEF/Visfatin (8X6)
  • 2 vials human PBEF/Visfatin Standard lyophilized, 50 ng/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human PBEF/Visfatin antibody
  • 2 vials Streptavidin-HRP solution
  • 4 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
• Background:

  PBEF (preB cell colonyenhancing factor. also visfatin and nicotinamide phosphoribosyltransferase) is a 52 kDa member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the Cterminal 128 amino acids (aa 364491). Over aa 27491, human PBEF shares 96%, 97%, and 96% aa identity with mouse, porcine, and canine PBEF, respectively.

• Gene Symbol: NAMPT
• Standard Curve:
  
  Representative standard curve for PBEF/Visfatin ELISA. PBEF/Visfatin was diluted in serial two-fold steps in Sample Diluent.