Human MMP-9 ELISA Kit (96-well)

CAT#: EA100106

Human MMP-9 ELISA Kit (96-well)

Product Images

Representative standard curve for MMP-9 ELISA. MMP-9 was diluted in serial two-fold steps in Sample Diluent.

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 62.5-4000pg/ml
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Human MMP-9 Ligand
• Sensitivity: 31pg/mL
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with antibody to human MMP-9 (8x12)
  • 1 vials human MMP-9 Standard lyophilized, 4000 pg/ml upon reconstitution
  • 1 vials concentrated Biotin-Conjugate anti-human MMP-9 antibody
  • 1 vials Streptavidin-HRP solution
  • 2 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 2 pieces Adhesive Films
  • package insert
• Background:

  Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade extracellular matrix proteins . They are secreted as zymogens (Pro-MMPs) that are activated by a variety of proteinases or by reaction with organic mercurials. They are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs) and by 2-macroglobulin . The regulation of MMP activity is important in tissue remodeling, inflammation, tumor growth and metastasis .

  Human MMP-9 (also known as gelatinase B) is secreted as a 92 kDa zymogen . Cleavage of Pro-MMP-9 at or near residue 87 results in the active enzyme with a mass of approximately 82 kDa . MMP-9 has three fibronectin type II domains, a hemopexin-like domain and a proline-rich type V collagen-homologous domain . Pro-MMP-9 can be activated by MMP-3 or by certain bacterial proteinases . MMP-9 is inhibited by.2-macroglobulin or by TIMP-1 , which binds to Pro-MMP-9 as well as to active MMP-9 . In vitro treatment of Pro-MMP-9 with 4-aminophenylmercuric acid (APMA) produces not only the 82 kDa active enzyme but also a C-terminal truncated form of approximately 65 kDa with the activity comparable to that of the 82 kDa form .

  Pro-MMP-9 is secreted by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts, chondrocytes, skeletal muscle satellite cells, endothelial cells, and various tumor cells(1, 7- 21). Pro-MMP-9 expression is upregulated by TGF-.1, IL-1., TGF-., PDGF-AB, TNF-., and IL-1. . Substrates for MMP-9 include denatured collagen type I (gelatin), native collagens type IV, V, VII, X and XI, fibrinogen, vitronectin, IL-1., and entactin, a molecule that bridges laminin and type IV collagen .

• Gene Symbol: MMP9
• Standard Curve:
  
  Representative standard curve for MMP-9 ELISA. MMP-9 was diluted in serial two-fold steps in Sample Diluent.