Human sE-selectin ELISA Kit (48-well)

CAT#: EA100111

Human sE-selectin ELISA Kit (48-well)

Product Images

Representative standard curve for sE-selectin ELISA. sE-selectin was diluted in serial two-fold steps in Sample Diluent.

Specifications

Product Data

• Format: 48-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 780-50000pg/ml
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Human sE-selectin Ligand
• Sensitivity: 200pg/mL
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Background:

  E-Selectin (also known as Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, or CD62E) is a 115 kDa, type I transmembrane glycoprotein expressed only on endothelial cells and only after activation by inflammatory cytokines (IL-1. or TNF-.) or endotoxins . Expression is transitory, reaching a maximum within about 6 hours of stimulation and then declining with shedding of soluble E-Selectin . Cell-surface E-Selectin is a mediator of the rolling attachment of leukocytes to the endothelium, an essential step in extravasation of leukocytes at the site of inflammation thereby playing a key role in localized inflammatory response. E-Selectin is believed to be particularly important in inflammation involving the skin .

  The extracellular part of E-Selectin includes a calcium-dependent C2-type lectin domain, an epidermal growth factor (EGF) domain, and six repeats of a complement-regulatory-protein-like sequence . E-Selectin binds sialyl Lewis X (sLex), a sialic acid-galactose-N-acetylglucosamine-fructose tetrasaccharide, but the actual recognition is thought to be for a specific presentation of those glycosyl units in a precise three-dimensional configuration on a specific glycoprotein rather than for that particular carbohydrate .

  Soluble E-Selectin (sE-Selectin) is found in the blood of healthy individuals , probably arising from proteolytic cleavage of the surface-expressed molecule . Elevated levels of sE-Selectin in serum have been reported in a variety of pathological conditions . Although it might be anticipated that sE-Selectin would suppress leukocyte migration by competing with surface-associated E-Selectin, it may actually activate neutrophils and act as a pro-inflammatory agent .

• Gene Symbol: SELE
• Standard Curve:
  
  Representative standard curve for sE-selectin ELISA. sE-selectin was diluted in serial two-fold steps in Sample Diluent.