Mouse IL-6 ELISA Kit (96-well)

CAT#: EA100128

Mouse IL-6 ELISA Kit (96-well)

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hour
• Signal: Colorimetric
• Curve Range: 0.02 - 1.0 ng/mL
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Mouse IL-6 Ligand
• Sensitivity: 7pg/mL
• Reactivities: Mouse
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with antibody to mouse IL-6 (8x12)
  • 2 vials mouse IL-6 Standard lyophilized, 2000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-mouse IL-6 antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
• Background:

  Interleukin 6 (IL-6) is a multifunctional cytokine that plays important roles in host defense, acute phase reactions, immune responses, nerve cell functions and hematopoiesis . It is expressed by a variety of normal and transformed lymphoid and nonlymphoid cells.

  IL-6 is a prototypic member of the IL-6 superfamily of cytokines that share gp130 as a component required for signal transduction . The mouse , rat and human IL-6 cDNAs have been cloned. The mouse IL-6 cDNA encodes a 211 amino acid (aa) residue precursor polypeptide with a hydrophobic signal sequence that is cleaved to generate the 187 aa residue mature protein. Mouse to rat and human, there is approximately 87% and 39% aa identity, respectively. Although human and mouse IL-6 are equally active on mouse cells, mouse IL-6 is not active on human cells .

  The high-affinity IL-6 receptor complex, which mediates IL-6 bioactivity, consists of two membrane glycoproteins. The production of IL-6 is upregulated by numerous signals such as mitogenic or antigenic stimulation, lipopolysaccharides, calcium ionophores, cytokines and viruses. IL-4, IL-10 and IL-13 inhibit IL-6 expression in monocytes. Elevated serum IL-6 levels have been observed in a number of pathological conditions, including bacterial and viral infections, trauma, autoimmune diseases, inflammations and malignancies .

• Gene Symbol: Il6
• Standard Curve:
  
  Representative standard curve for IL-6 ELISA. IL-6 was diluted in serial two-fold steps in Sample Diluent.