Mouse IL-12p70 ELISA Kit (96-well)

CAT#: EA100130

Mouse IL-12p70 ELISA Kit (96-well)

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hour
• Signal: Colorimetric
• Curve Range: 0.02 - 1.0 ng/mL
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Mouse IL-12p70 Ligand
• Sensitivity: 7pg/mL
• Reactivities: Mouse
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with monoclonal antibody to mouse IL-12p70 (8X12)
  • 2 vials mouse IL-12p70 Standard lyophilized, 2000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-mouse IL-12p70 antibody
  • 2 vials Streptavidin-HRP solution,
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 3 pieces Adhesive Films
  • package insert
• Background:

  Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of a human B-lymphoblastoid cell line .

  Biologically active mouse IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. While the p40 and p35 subunits by themselves do not have IL-12 activity, the p40 homodimer has been shown to bind the IL-12 receptor and is an IL-12 antagonist . Mature mouse p35 subunit is composed of 193 amino acid (aa) residues and contains seven cysteines plus one potential N-linked glycosylation site . Mature mouse p40 subunit has 313 aa, with 13 cysteines and five potential N-linked glycosylation sites . Mouse p35 and p40 subunits show 63% and 72% aa identity, respectively, to the human p35 and p40 subunits . Although mouse IL-12 is active on both human and mouse cells, human IL-12 is only active on human cells.

  IL-12 has been shown to have multiple effects on T lymphocytes and natural killer (NK) cells. Some of these effects include the induction of IFN-. and TNF production by T and NK cells, the enhancement of cytotoxic activity of T and NK cells and the stimulation of T and NK cell proliferation. IL-12 has also been shown to be a central mediator of the cell-mediated immune response by promoting Th1 development .

  Cell surface staining for IL-12 on a human monocytic and a mouse macrophage cell line has been reported, suggesting that membrane-associated IL-12 may exist . Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. Although a human B cell line has been shown to produce IL-12, fresh B cells are apparently not producers of IL-12.

• Gene Symbol: Il12b
• Standard Curve:
  
  Representative standard curve for IL-12p70 ELISA. IL-12p70 was diluted in serial two-fold steps in Sample Diluent.