Mouse VEGF ELISA Kit (96-well)

CAT#: EA100132

Mouse VEGF ELISA Kit (96-well)

Product Images

Representative standard curve for VEGF ELISA. VEGF was diluted in serial two-fold steps in Sample Diluent.

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hour
• Signal: Colorimetric
• Curve Range: 0.02 - 1.0 ng/mL
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Mouse VEGF Ligand
• Sensitivity: 7pg/mL
• Reactivities: Mouse
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with antibody to mouse VEGF (8 x 12)
  • 2 vials mouse VEGF Standard lyophilized, 2000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-mouse VEGF antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
• Background:

  Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adults . It is a member of the plateletderived growth factor family that is characterized by a cysteine knot structure formed by eight conserved cysteine residues . Mouse embryos expressing only the VEGF120 isoform do not survive to term and show defects in skeletogenesis . Mouse VEGF120 shares 98% aa sequence identity with corresponding regions of rat, 89% with canine, feline, equine and porcine, and 87% with human, ovine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt 1) and VEGF R2 (Flk1/KDR) on endothelial cells . VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells . In adults, VEGF functions mainly in wound healing and the female reproductive cycle . Pathologically, it is involved in tumor angiogenesis and vascular leakage . Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus .VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-. .

• Gene Symbol: Vegfa
• Standard Curve:
  
  Representative standard curve for VEGF ELISA. VEGF was diluted in serial two-fold steps in Sample Diluent.