Mouse MIP-2 ELISA Kit (48-well)

CAT#: EA100137

Mouse MIP-2 ELISA Kit (48-well)

Specifications

Product Data

• Format: 48-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hour
• Signal: Colorimetric
• Curve Range: 0.02 - 1.0 ng/mL
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Mouse MIP-2 Ligand
• Sensitivity: 7pg/mL
• Reactivities: Mouse
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with antibody to mouse MIP-2 (8 6)
  • 2 vials mouse MIP-2 Standard lyophilized, 500 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-mouse MIP-2 antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
• Background:

  Mouse macrophage inflammatory protein-2 (MIP-2), also known as CXCL2, was originally identified as a heparin-binding protein secreted by an LPS-stimulated mouse macrophage cell line . A cDNA clone encoding the protein was isolated from this cell line and characterized . Based on its protein and DNA sequences, mouse MIP-2 was classified as a member of the alpha (CXC) chemokine family of inflammatory and immunoregulatory cytokines .

  Mouse MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature mouse MIP-2. The protein sequence of mouse MIP-2 shows approximately 63% identity to that of mouse KC, another mouse alpha chemokine. Mouse MIP-2 is also 60% identical to human GRO. and GRO.. Based on these protein sequence similarities, it is likely that mouse KC and MIP-2 are homologs of human GRO., . and . chemokines. Since chemokines with protein sequence homology to human IL-8 have not been identified in mice, it has been suggested that the mouse KC and MIP-2 are functional homologs of human IL-8 in mice . A putative mouse homolog of the human IL-8 receptor beta (IL-8 R.) has also been cloned. This receptor shows 71% identity to human IL-8 R. and 68% identity to human IL-8 R.. Both mouse KC and MIP-2 bind mouse IL-8 R. with high affinity .

  Like human IL-8, mouse MIP-2 exhibits potent neutrophil chemotactic activity and may be a key mediator of neutrophil recruitment in response to tissue injury and infection . Increased MIP-2 expression has been found to be associated with neutrophil influx in various inflammatory conditions .

• Gene Symbol: Cxcl2
• Standard Curve:
  
  Representative standard curve for MIP-2 ELISA. MIP-2 was diluted in serial two-fold steps in Sample Diluent.