Mouse IL-1a ELISA Kit (48-well)

CAT#: EA100139

Mouse IL-1a ELISA Kit (48-well)

Size: 1 x 48 wells 1 x 96 wells


USD 290.00

3 Weeks*

Size
    • 1 x 48 wells

Product Images

Other products for "Il1a"

Specifications

Product Data
Format 48-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hour
Signal Colorimetric
Curve Range 1.8 - 125 pg/mL
Sample Type Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume 20 uL
Specificity Natural and recombinant Mouse IL-1a Ligand
Sensitivity 1pg/mL
Reactivities Mouse
Interference No significant interference observed with available related molecules.
Components
  • Aluminium pouches with a Microwell Plate coated with antibody to mouse IL-1a (8 6)
  • 2 vials mouse IL-1a Standard lyophilized, 250 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-mouse IL-1a antibody
  • 2 vials Streptavidin-HRP solution,
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 3 pieces Adhesive Films
  • package insert
Background

Interleukin-1 alpha(IL-1a) is a proinflammatory cytokine produced by a wide variety of cell types, including macrophages, osteoblasts, monocytes and hepatocytes. IL-1 alpha is synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17 kDa. The IL-1a precursor reportedly shows full biological activity in the EL-4 assay. Circulating levels of IL-1a are normally low and only rise after stimulation by agents such as those produced byinflammation, infection or microbial endotoxins. IL-1a possesses a wide variety of biological activities and exerts its effects by binding to specific cell surface receptors.

Gene Symbol Il1a
Standard Curve
Representative standard curve for IL-1a ELISA. IL-1a was diluted in serial two-fold steps in Sample Diluent.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.