Mouse TGF-beta1 ELISA Kit (48-well)

CAT#: EA100141

Mouse TGF-beta1 ELISA Kit (48-well)

Specifications

Product Data

• Format: 48-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hour
• Signal: Colorimetric
• Curve Range: 0.03 - 2.0 ng/mL
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Mouse TGF-β1 Ligand
• Sensitivity: 15pg/mL
• Reactivities: Mouse
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with antibody to mouse TGF-β1 (8 6)
  • 2 vials mouse TGF-β1 Standard lyophilized, 2000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-mouse TGF-β1 antibody
  • 2 vials Streptavidin-HRP solution,
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 2 vial 1N HCl
  • 2 vial 1.2 N NaOH/0.5 M HEPES
  • 2 vial 2.7N NaOH/1 M HEPE
  • 2 vial 2.5N Acetic Acid/10 M Urea
  • 3 pieces Adhesive Films
  • package insert
• Background:

  Transforming Growth Factor-beta (TGF-.) is a pleiotropic cytokine which exists in five isoforms, known as TGF-.1-5. TGF-.1 is the most abundant form in lymphoid organs and is found almost ubiquitously while other isoforms are expressed in a more restricted distribution. The biologically active forms of all isoforms are disulfide-linked homodimers. The heat- and acid- stable monomeric subunits have a length of 112 amino acids. TGF-.1 is produced in very high levels by platelets. Other cellular sources of TGF-.1 include macrophages, lymphocytes, endothelial cells, chondrocytes, and leukemic cells. TGF-.1 secretion can be induced by steroids, retinoids, EGF, NGF, vitamin D3, and IL-1. Activities of TGF-.1 include inhibition of cell growth for inhibitor for normal and transformed epithelial cells, endothelial cells, fibroblasts, neurons, and lymphoid cells and other hematopoietic cell types. TGF-.1 inhibits the proliferation of T cells and NK cells and down-regulates the activities of activated macrophages. TGF-.1 blocks the anti-tumor activity of IL-2-bearing lymphokine-activated killer (LAK) cells. TGF-.1 has a critical role in the development of regulatory T cells. Dendritic cells exposed to tumors have been reported to secrete TGF-.1 and stimulate expansion of naturally-occurring T reg cells. Moreover, TGF-.1 has been shown to act as a costimulatory factor for expression of Foxp3, leading to the differentiation of CD4+CD25+ Treg cells from peripheral CD4+CD25- progeny. TGF-.-induced regulatory T cells have been termed Ti-Treg.

• Gene Symbol: Tgfb1
• Standard Curve:
  
  Representative standard curve for TGF-ß1 ELISA. TGF-ß1 was diluted in serial two-fold steps in Sample Diluent.