Mouse IL-12/IL-23(P40) ELISA Kit (96-well)

CAT#: EA100146

Mouse IL-12/IL-23(P40) ELISA Kit (96-well)

Size: 1 x 48 wells 1 x 96 wells


USD 415.00

3 Weeks*

Size
    • 1 x 96 wells

Product Images

Other products for "Il12b"

Specifications

Product Data
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hour
Signal Colorimetric
Curve Range 7.8 - 500 pg/mL
Sample Type Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume 20 uL
Specificity Natural and recombinant Mouse IL-12/IL-23 (P40) Ligand
Sensitivity 4pg/mL
Reactivities Mouse
Interference No significant interference observed with available related molecules.
Components
  • Aluminium pouches with a Microwell Plate coated with antibody to mouse IL-12/IL-23p40 (8X12)
  • 2 vials mouse IL-12/IL-23p40 Standard lyophilized, 1000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-mouse IL-12/IL-23p40 antibody
  • 2 vials Streptavidin-HRP solution,
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 3 pieces Adhesive Films
  • package insert
Background

IL-12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine produced primarily by antigen-presenting cells (monocytes/macrophages, dendritic cells and B lymphocytes). IL-12 has multiple effects on T lymphocytes and natural killer (NK) cells, including the ability to stimulate cytotoxicity, proliferation, cytokine production and Th1 subset development . IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a unique 35 kDa (p35) subunit and a common 40 kDa (p40) subunit that is also present in IL-23. Monomers of the p40 and p35 subunits by themselves do not have IL-12 activity, but the homodimer of p40 has been shown to bind the IL-12 receptor and is an IL-12 antagonist . In cells expressing both p35 and p40 mRNAs, p40 mRNA is expressed to a higher level and free p40 subunits not associated with p35 subunits are secreted together with heterodimeric IL-12 p70 . Most of the free p40 subunits secreted by the various human cell lines examined have been found to exist as monomers . In the culture supernates of various activated human monocytes where free p40 is present in vast excess over p70, the levels of p70 measured by bioassays are consistent with those measured using a p70-specific immunoassay, suggesting that p40 monomers are not efficient IL-12 antagonists . In the mouse system, p40 homodimers are produced in vivo and function as IL-12 antagonists . Polymorphisms exist in the mouse IL-12/IL-23 p40 sequence.

Gene Symbol Il12b
Standard Curve
Representative standard curve for IL-12/IL-23p40 ELISA. IL-12/IL-23p40 was diluted in serial two-fold steps in Sample Diluent.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.