Rat IFN-gamma ELISA Kit (96-well)

CAT#: EA100162

Rat IFN-gamma ELISA Kit (96-well)

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hour
• Signal: Colorimetric
• Curve Range: 0.03 - 2.0 ng/mL
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Rat IFN-? Ligand
• Sensitivity: 7pg/mL
• Reactivities: Rat
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with monoclonal antibody to rat IFN-? (8X12)
  • 2 vials human IFN-? Standard lyophilized, 20000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human IFN-? antibody
  • 2 vials Streptavidin-HRP solution,
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 3 pieces Adhesive Films
  • package insert
• Background:

  Interferon gamma (IFN-.) is a multifunctional protein first observed as an antiviral activity in cultures of Sindbis virus-infected human leukocytes stimulated by PHA . Produced by Tlymphocytes and natural killer (NK) cells, IFN-. ?is now known to be both an inhibitor of viral replication and a regulator of numerous immunological functions. Human IFN-.?is reported to be active only on human and non-human primate cells . The biochemistry and biological activities of the interferons have been extensively reviewed .

  Rat IFN-. cDNA encodes a 156 amino acid (aa) residue precursor protein with a putative 19 aa residue signal peptide that is cleaved to generate the mature protein which contains two potential N-glycosylation sites . Rat IFN-. shares approximately 87% and 39% amino acid sequence identity with mouse IFN-. and human IFN-. , respectively. Consistent with their degrees of shared homology, rat A receptor for IFN-.?has been identified and its gene localized to chromosome 6 . Apparently the product of a single gene, the receptor is a single chain 90 kDa glycoprotein that shows a high degree of species-specific binding of IFN-.?.

  Functionally, IFN-. ?produces a variety of effects. Produced by CD8+, NK, gd,and TH1 T helper cells, IFN-. ?has documented antiviral, antiprotozoal and immunomodulatory effects on cell proliferation and apoptosis, as well as the stimulation and repression of a variety of genes he antiprotozoal activity of IFN-. ?against Toxoplasma and Chlamydia is believed to result from indoleamine 2,3-dioxygenase activity, an enzyme induced by IFN-. ?.The immunomodulatory effects of IFN-.? are extensive and diverse. In monocyte/macrophages, the activities of IFN-.?include: increasing the expression of class I and II MHC antigens; increasing the production of IL-1, platelet-activating factor, H2O2, and pterin; protection of monocytes against LAK cell-mediated lysis; downregulation of IL-8 mRNA expression that is upregulated by IL-2; and, with lipopolysaccharide, induction of NO production.Finally, IFN-.?has been shown to upregulate ICAM-1, but not E-Selectin or VCAM-1, expression on endothelial cells.

• Gene Symbol: Ifng
• Standard Curve:
  
  Representative standard curve for IFN-? ELISA. IFN-? was diluted in serial two-fold steps in Sample Diluent.