Rat TNF-a ELISA Kit (96-well)
USD 415.00
3 Weeks*
Product Images
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Specifications
Product Data | |
Format | 96-well strip plate |
Assay Type | Solid Phase Sandwich ELISA |
Assay Length | 3 hour |
Signal | Colorimetric |
Curve Range | 0.03 - 2.0 ng/mL |
Sample Type | Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) |
Sample Volume | 20 uL |
Specificity | Natural and recombinant Rat TNF-a Ligand |
Sensitivity | 15pg/mL |
Reactivities | Rat |
Interference | No significant interference observed with available related molecules. |
Components |
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Background | The prototype ligand of theTNFsuperfamily, TNF-./TNFSF1A, is a pleiotropic cytokine that plays a central role in inflammation and apoptosis .TNF-. is produced by activated macrophages and other cell types including T and B cells, NK cells, LAK cells, astrocytes, endothelial cells, smooth muscle cells and some tumor cells . Rat TNF-. cDNA encodes a 235 amino acid (aa) residue type II membrane protein . The 156 aa residue soluble TNF-.is released from the C-terminus of the membrane-anchored TNF-. by TNF-.-converting enzyme (TACE), a matrix metalloprotease . The membrane-anchored form of TNF-. has been shown to have lytic activity and may also have an important role in intercellular communication . The biologically active TNF-. has been shown to exist as a trimer .TNF-. is reported to promote inflammatory cell infiltration by upregulating leukocyte adhesion molecules on endothelial cells, serve as a chemotactic agent for monocytes, and activate phagocyte killing mechanisms . Deficiencies in either TNF-. or its receptors can increase susceptibility to infection by intracellular pathogens . TNF-. may also play a role in lymphoid tissue development. Knockout mice lack splenic B cell follicles and the ability to form germinal centers . Other potential physiological roles for TNF-. and its receptors include regulating the differentiation of hematopoietic stem and progenitor cells . |
Gene Symbol | Tnf |
Standard Curve |
![]() Representative standard curve for TNF-a ELISA.TNF-a was diluted in serial two-fold steps in Sample Diluent.
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Documents
SDS |
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