Rat IL-10 ELISA Kit (96-well)

CAT#: EA100170

Rat IL-10 ELISA Kit (96-well)

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hour
• Signal: Colorimetric
• Curve Range: 0.03 - 2.0 ng/mL
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Rat IL-10 Ligand
• Sensitivity: 15pg/mL
• Reactivities: Rat
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with monoclonal antibody to rat IL-10 (8X12)
  • 2 vials rat IL-10 Standard lyophilized, 2000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-rat IL-10 antibody
  • 2 vials Streptavidin-HRP solution,
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 3 pieces Adhesive Films
  • package insert
• Background:

  Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitor, is a pleiotropic cytokine that inhibits the production of a number of cytokines (including IL-1, GM-CSF, TNF, IL-6, IL-8, IL-12 and IFN-.) by activated Th1 cells, NK cells, and monocytes/macrophages.

  Rat IL-10 cDNA encodes a 178 amino acid (aa) residue precursor protein that contains an 18 aa signal sequence and a 160 aa mature protein . Within the mature protein, there are two potential N-linked glycosylation sites and five cysteine residues. Mature rat IL-10 shares 85% and 74% aa sequence identity with mouse and human IL-10, respectively . Although rat and human IL-10 are both active on mouse cells, mouse IL-10 is not active on human cells .

  Based on human and mouse studies, biologically active rat IL-10 is likely to be a nondisulfide-linked homodimer in solution . Although IL-10 is considered to be a strictly secreted cytokine, the existence of biologically-active, membrane-bound IL-10 has also been reported. IL-10 has been shown to inhibit macrophage cytotoxic activity and to stimulate the proliferation and differentiation of B cells, mast cells, and thymic T cells. IL-10 is a potent modulator of monocyte/macrophage function. IL-10 also enhances the release of soluble TNF receptors and inhibits the expression of surface ICAM-1 and B7 .

  Finally, IL-10 has been reported to suppress the synthesis of superoxide anion plus reactive oxygen intermediates (ROI), and either inhibit or facilitate nitric oxide synthesis, depending on the time of exposure to activated macrophages . IL-10 has marked effects on B cells. IL-10 also has documented activity on endothelial cells, where it mimics IL-4 , and on thymocytes and mast cells, where it acts as a growth costimulator .

• Gene Symbol: Il10
• Standard Curve:
  
  Representative standard curve for IL-10 ELISA. IL-10 was diluted in serial two-fold steps in Sample Diluent.