Rat MIP-2 ELISA Kit (96-well)

CAT#: EA100174

Rat MIP-2 ELISA Kit (96-well)

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hour
• Signal: Colorimetric
• Curve Range: 0.03 - 2.0 ng/mL
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Rat MIP-2 Ligand
• Sensitivity: 15pg/mL
• Reactivities: Rat
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with monoclonal antibody to rat MIP-2 (8X12)
  • 2 vials rat MIP-2 Standard lyophilized,4000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-rat MIP-2 antibody
  • 2 vials Streptavidin-HRP solution,
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 3 pieces Adhesive Films
  • package insert
• Background:

  Macrophage inflammatory protein-2(MIP-2) was originally identified as a heparinbinding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. The protein is produced by a variety of cell types, including intestinal epithelium, macrophages, astrocytes and fibroblasts. Homologs of the mouse MIP-2 have been identified in human, rat and cotton rat. MIP-2 is an ELR-containing member of the alpha (CXC) subfamily of chemokines. The cotton rat MIP-2 gene encodes a 101 amino acid (aa) residue precursor protein with a 28 aa putative signal peptide that is cleaved to generate the 73 aa mature protein. Mature cotton rat MIP-2 has two intrachain disulfide bonds and no potential glycosylation sites. It shares approximately 79% and 85% aa sequence identity with rat CINC-3 and mouse MIP-2, respectively. MIP-2 is a potent neutrophil attractant and activator. MIP-2 binds and activates the chemokine receptor CXCR-2. The recombinant cotton rat MIP-2 has been shown to bind and activate the human CXCR-2.

• Gene Symbol: Cxcl2
• Standard Curve:
  
  Representative standard curve for MIP-2 ELISA. MIP-2 was diluted in serial two-fold steps in Sample Diluent.