Rat TGF-beta1 ELISA Kit (48-well)

CAT#: EA100177

Rat TGF-beta1 ELISA Kit (48-well)

Specifications

Product Data

• Format: 48-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hour
• Signal: Colorimetric
• Curve Range: 0.06 - 4.0 ng/mL
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Rat TGF-β1 Ligand
• Sensitivity: 15pg/mL
• Reactivities: Rat
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with antibody to rat TGF-β1 (8x6)
  • 2 vials rat TGF-β1 Standard lyophilized, 2000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-rat TGF-β1 antibody
  • 2 vials Streptavidin-HRP solution
  • 4 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 2 vial 1N HCl
  • 2 vial 1.2 N NaOH/0.5M HEPES
  • 4 pieces Adhesive Films
  • package insert
• Background:

  Transforming growth factor (TGF), a 'factor' that promoted the transformation of cultured fibroblasts into a tumor-like phenotype, was subsequently found to be more of a tumor suppressor than tumor promoter and to be a mixture of two proteins, TGF-. and TGF-.. These molecules are members of a superfamily that includes TGF-.1 through 5, bone morphogenic proteins, activins and inhibins. It plays a critical role in cellular growth, development, differentiation, proliferation, extracellular matrix (ECM) synthesis and degradation, control of mesenchymal-epithelial interactions during embryogenesis, immune modulation, apoptosis, cell cycle progression, angiogenesis, adhesion and migration and leukocyte chemotaxis.

  Originally, TGF-.1 was separated from platelets and later found that TGF-.1 can be expressed in many organizations. Human TGF-.1 is a 25kDa, disulfide-linked, non-glycosylated homodimer. Biological activity of TGF-.1 is regulated by a number of receptors, including receptor I (53-65KD), receptor II (83-110KD), receptor III (250-310KD), receptor-IV (60KD) and receptor V (400KD).

  TGF-.1 is the key mediator in the pathophysiology of tissue repair and human fibrogenesis: balance between production and degradation of type I collagen, and fibrosis and scarring in organ and tissue. TGF-.1 exhibits important immunoregulatory features of partially adverse character: TGF-.1 inhibits B and T cell proliferation, differentiation and antibody production as well as maturation and activation of macrophages. TGF-.1 is synthesized, with only a few exceptions, by virtually all cells, and TGF receptors are expressed by all cells. TGF-. affects nearly every physiological process in some way; its systemic and cell-specific activities are too complicated to review here. There are, however, three fundamental activities: TGF-.1 modulates cell proliferation, generally as a suppressor; TGF-.1 enhances the deposition of extracellular matrix through promotion of synthesis and inhibition of degradation; TGF-.1 is immunosuppressive through a variety of mechanisms. The specific action of TGF-. on a particular cell depends on the exact circumstances of that cell's environment.

• Gene Symbol: Tgfb1
• Standard Curve:
  
  Representative standard curve for TGF-ß1 ELISA. TGF-ß1 was diluted in serial two-fold steps in Sample Diluent.