Rat IL-1a ELISA Kit (48-well)

CAT#: EA100179

Rat IL-1a ELISA Kit (48-well)

Specifications

Product Data

• Format: 48-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hour
• Signal: Colorimetric
• Curve Range: 0.02 - 1.0 ng/mL
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Rat IL-1a Ligand
• Sensitivity: 7pg/mL
• Reactivities: Rat
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with monoclonal antibody to rat IL-1a (8x6)
  • 2 vials rat IL-1a Standard lyophilized, 1000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-rat IL-1a antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
• Background:

  Interleukin-1. (IL-1., also known as IL-1F1) and IL-1. (IL-1F2) are pleiotropic cytokines that belong to the IL-1 gene family. IL-1. and IL-1. bind to the same cell surface receptors and share biological functions. The two proteins have approximately 23% amino acid (aa) sequence homology and both are synthesized as 31 kDa precursors that lack hydrophobic signal peptide sequences. Current evidence suggests that IL-1 proteins may be secreted via non-classical pathways .

  Rat IL-1. cDNA encodes a 270 aa residue pro-IL-1. precursor . The 114 aa pro-region contains a nuclear localization sequence, a lysine-based myristoylation site, one phosphorylation site, and one potential N-linked glycosylation site . The 156 aa mature region contains no cysteines and one potential N-linked glycosylation site . Pro-IL-1. is primarily localized to the cytosol after synthesis. It is known to translocate to the nucleus after cell activation and initiate gene transcription . Some IL-1. is released extracellularly and exists as either a membrane-bound form or as a circulating 17 kDa molecule. When membranebound, membrane association is mediated either via a poorly-understood cell surface lectin interaction with the glycosylated IL-1. pro-form, or by interaction of myristoylated pro-IL-1. with plasma membrane phospho-lipids . When released as a 17 kDa soluble form, calpain initiates cleavage of the pro-IL-1. precursor . Unlike pro-IL-1., which is biologically inactive, both pro-IL-1. and mature IL-1. have been shown to be biologically active . Within the mature protein, rat IL-1. shares 79%, 60%, 59%, 58%, 57%, and 56% aa sequence identity with mouse, rabbit, human, bovine, canine and feline proteins, respectively . Mammalian cells known to express IL-1. include brown adipocytes , keratinocytes , monocytes , macrophages , endothelial and smooth muscle cells , mast cells , Schwann cells , as well as osteoblasts and osteoclasts .

  Three type I transmembrane immunoglobulin superfamily proteins, IL-1 receptor type I (IL-1 R1), IL-1 receptor type II (IL-1 R2), and IL-1 receptor accessory protein (IL-1 RAcP, IL-1 R3)are involved in the formation of high affinity cell surface IL-1 receptor complexes. Both IL-1 R1 and IL-1 R2 can bind directly to IL-1.. IL-1 RAcP does not bind IL-1. directly, but interacts with IL-1 R1 in the presence of IL-1 to form the high-affinity receptor complex which is required for intracellular signal transduction. IL-1 RAcP also interacts with IL-1 R2 to form a non-functional high-affinity receptor complex that does not transduce IL-1 signals. Therefore, IL-1 R2 functions as a decoy receptor that attenuates IL-1. functions .

  IL-1. possesses a wide variety of biological activities and plays a central role in mediating immune and inflammatory responses. Normal production of IL-1. is critical for hematopoiesis, angiogenesis, osteoclast differentiation, and initiation of normal host responses to injury and infection(15,32-34). Inappropriate production of IL-1. has been implicated in the production of a variety of pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, insulin-dependent diabetes mellitus, and atherosclerosis .

• Gene Symbol: Il1a
• Standard Curve:
  
  Representative standard curve for IL-1a ELISA. IL-1a was diluted in serial two-fold steps in Sample Diluent.