Rat GM-CSF ELISA Kit (48-well)
USD 290.00
3 Weeks*
Product Images
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Specifications
Product Data | |
Format | 48-well strip plate |
Assay Type | Solid Phase Sandwich ELISA |
Assay Length | 3 hour |
Signal | Colorimetric |
Curve Range | 0.02 - 1.0 ng/mL |
Sample Type | Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) |
Sample Volume | 20 uL |
Specificity | Natural and recombinant Rat GM-CSF Ligand |
Sensitivity | 7pg/mL |
Reactivities | Rat |
Interference | No significant interference observed with available related molecules. |
Components |
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Background | GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocytemacrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils . GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity . It shows clinical effectiveness in ameliorating chemotherapyinduced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines . The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled .helices. Mature rat GM-CSF shares 56-69% amino acid sequence identity with canine, feline, human, mouse, and porcine GM-CSF. GM-CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R./CD116 and the signal transducing common . chain (CD131) which is also a component of the highaffinity receptors for IL-3 and IL-5 . In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R. . Rat GM-CSF is active on mouse cells, although mouse GM-CSF is only weakly active on rat cells . |
Gene Symbol | Csf2 |
Standard Curve |
![]() Representative standard curve for GM-CSF ELISA. GM-CSF was diluted in serial two-fold steps in Sample Diluent.
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Documents
SDS |
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