Human Fractalkine/CX3CL1 ELISA Kit

CAT#: EA100231

Human Fractalkine/CX3CL1 ELISA Kit

Product Images

Representative standard curve for Human Fractalkine/CX3CL1 PicoKine ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human Fractalkine/CX3CL1
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 156pg/ml-10,000pg/ml
• Sample Type: Cell culture supernates, serum and plasma(heparin, EDTA), saliva, urine and human milk.
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human Fractalkine
• Sensitivity: <50pg/ml
• Reactivities: Human
• Cross Reactivity: There is detectable cross-reactivity with Eotaxin, MCP-1, MCP-2, 6Ckine, MARC< 0.2%.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human Fractalkine/CX3CL1 antibody 1
  • Lyophilized recombinant human Fractalkine/CX3CL1 tandard 10ng/tubex2
  • Biotinylated anti- human Fractalkine/CX3CL1 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: Chemokine(C-X3-C motif) ligand 1(CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine(in humans) and neurotactin(in mice). CX3CL1 is produced as a long protein(with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble(90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22. It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.
• Gene Symbol: CX3CL1
• Standard Curve:
  
  Representative standard curve for Human Fractalkine/CX3CL1 PicoKine ELISA Kit