Rat IGF-2 ELISA Kit

CAT#: EA100252

Rat IGF-2 ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Rat IGF-2
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 62.5pg/ml-4000pg/ml
• Sample Type: Cell culture supernates, serum and plasma(heparin, EDTA).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of rat IGF-2
• Sensitivity: <5pg/ml
• Reactivities: Rat
• Cross Reactivity: There is no detectable cross-reactivity with IGF-1.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- rat IGF-2 antibody 1
  • Lyophilized recombinant rat IGF-2 standard 10ng/tubex2
  • Biotinylated anti- rat IGF-2 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.
• Gene Symbol: Igf2
• Standard Curve:
  
  Representative standard curve for Rat IGF-2 PicoKine ELISA Kit