Mouse IGF-2 ELISA Kit

CAT#: EA100253

Mouse IGF-2 ELISA Kit


USD 500.00

3 Weeks*

Size
    • 1 x 96 wells

Product Images

Other products for "Igf2"

Specifications

Product Data
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse IGF-2
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 62.5pg/ml-4000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of mouse IGF-2
Sensitivity <5pg/ml
Reactivities Mouse
Cross Reactivity There is no detectable cross-reactivity with IGF-1.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- mouse IGF-2 antibody 1
  • Lyophilized recombinant mouse IGF-2 standard 10ng/tubex2
  • Biotinylated anti- mouse IGF-2 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.
Gene Symbol Igf2
Standard Curve
Representative standard curve for Mouse IGF-2 PicoKine ELISA Kit

Documents

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.