Human IL-12(p40) ELISA Kit

CAT#: EA100289

Human IL-12(p40) ELISA Kit

Product Images

Representative standard curve for Human IL-12(p40) PicoKine ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human IL-12(p40)
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 31.2pg/ml-2000pg/ml
• Sample Type: Cell culture supernates, serum and plasma(heparin, EDTA, citrate).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human IL-12(p40)
• Sensitivity: <2pg/ml
• Reactivities: Human
• Cross Reactivity: There is cross-reactivates with IL-12(p70), IL-23(p19/p40)< 5%.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human IL-12(P40) antibody 1
  • Lyophilized recombinant human IL-12(P40) standard 10ng/tubex2
  • Biotinylated anti- human IL-12(P40) antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: Interleukin-12(IL12; formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits. The larger 40-kDa subunit(p40) is a member of the cytokine receptor family, and the smaller 35-kDa subunit(p35) is related to IL6 and GCSF. Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p70 heterodimer. Interleukin(IL)-12 has been cloned on the basis of its ability to activate natural killer(NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon(IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection; for the development of a Th1 response, critical for effective host defense against intracellular pathogens; and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype. The subunits map to different chromosomes: p40(IL12B) to 5q31-q33 and p35(IL12A) to 3p12-3q13.2.2 The standard product used in this kit is recombinant human IL-12(p40), which is a 40KDa glycoprotein consisting of 306 amino acids.
• Gene Symbol: IL12B
• Standard Curve:
  
  Representative standard curve for Human IL-12(p40) PicoKine ELISA Kit