Rat NGF/NGF beta ELISA Kit

CAT#: EA100329

Rat NGF/NGF beta ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Rat NGF/NGF beta
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 15.6pg/ml-1000pg/ml
• Sample Type: Cell culture supernates and serum.
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of rat NGF
• Sensitivity: <1pg/ml
• Reactivities: Rat
• Cross Reactivity: There is no detectable cross-reactivity with BDNF, GDNF, CNTF, NT3, and NT4.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- rat NGF/NGF beta antibody 1
  • Lyophilized recombinant rat NGF/NGF beta standard 10ng/tubex2
  • Biotinylated anti- rat NGF/NGF beta antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.
• Gene Symbol: Ngf
• Standard Curve:
  
  Representative standard curve for Rat NGF/NGF beta PicoKine ELISA Kit