Rat Neurotrophin-3 ELISA Kit

CAT#: EA100332

Rat Neurotrophin-3 ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Rat Neurotrophin-3
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 15.6pg/ml-1000pg/ml
• Sample Type: Cell culture supernates and serum.
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of rat Neurotrophin-3
• Sensitivity: <2pg/ml
• Reactivities: Rat
• Cross Reactivity: There is cross-reactivates with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- rat Neurotrophin-3 antibody 1
  • Lyophilized recombinant rat Neurotrophin-3 standard 10ng/tubex2
  • Biotinylated anti- rat Neurotrophin-3 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.
• Gene Symbol: Ntf3
• Standard Curve:
  
  Representative standard curve for Rat Neurotrophin-3 PicoKine ELISA Kit