Human TGF alpha ELISA Kit

CAT#: EA100354

Human TGF alpha ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human TGF alpha
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 15.6pg/ml-1000pg/ml
• Sample Type: Cell culture supernates, serum, plasma(heparin, EDTA) and human milk.
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human TGF alpha
• Sensitivity: <1pg/ml
• Reactivities: Human
• Cross Reactivity: There is cross-reactivates with TGF beta 2, TGF beta 3, TGF beta 5<1%.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human TGF alpha antibody 1
  • Lyophilized recombinant human TGF alpha standard 10ng/tubex2
  • Biotinylated anti- human TGF alpha antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: Transforming growth factor alpha(TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene(TGFA) maps to human chromosome 2 close to the breakpoint of the t(2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-alpha showed similar immunoreactivity when compared with rat TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.
• Gene Symbol: TGFA
• Standard Curve:
  
  Representative standard curve for Human TGF alpha PicoKine ELISA Kit