Rat TGF beta 1 ELISA Kit

CAT#: EA100356

Rat TGF beta 1 ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Rat TGF beta 1
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 15.6pg/ml-1000pg/ml
• Sample Type: Cell culture supernates, serum, plasma(EDTA) and urine.
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of activated rat TGF beta 1
• Sensitivity: <1pg/ml
• Reactivities: Rat
• Cross Reactivity: There is cross-reactivates with TGFbeta2, TGFbeta3, TGFbeta5<1%.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- rat TGF beta 1 antibody 1
  • Lyophilized recombinant rat TGF beta 1 standard 10ng/tubex2
  • Biotinylated anti- rat TGF beta 1 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: Transforming growth factor-beta1(TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects. TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation. TGFbeta1 is also an important mediator of bone remodeling. TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation. TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide(beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.
• Gene Symbol: Tgfb1
• Standard Curve:
  
  Representative standard curve for Rat TGF beta 1 PicoKine ELISA Kit