Human VEGF ELISA Kit

CAT#: EA100376

Human VEGF ELISA Kit


USD 395.00

3 Weeks*

Size
    • 1 x 96 wells

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Other products for "VEGFA"

Specifications

Product Data
Assay Type Sandwich ELISA kit of Quantitative Detection for Human VEGF
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 31.2pg/ml-2000pg/ml(humanserum,plasma)15.6pg/ml-1000pg/ml(cellculturesupernates)
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human VEGF
Sensitivity <1pg/ml
Reactivities Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- human VEGF antibody 1
  • Lyophilized recombinant human VEGF standard 10ng/tubex2
  • Biotinylated anti- human VEGF antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Vascular permeability factor/vascular endothelial growth factor(VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis.VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy.VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.
Gene Symbol VEGFA

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.