Mouse Gp130/IL6ST ELISA Kit

CAT#: EA100398

Mouse Gp130/IL6ST ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Mouse Gp130/IL6ST
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 125pg/ml-8000pg/ml
• Sample Type: Cell culture supernates, serum and plasma(heparin, EDTA, citrate).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of mouse Gp130
• Sensitivity: <10pg/ml
• Reactivities: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- mouse Gp130/IL6ST antibody 1
  • Lyophilized recombinant mouse Gp130/IL6ST standard 10ng/tubex2
  • Biotinylated anti- mouse Gp130/IL6ST antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: GP130(Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membrane-bound gp130. Gp130 is an important part of many different types of signaling complexes.Gp130 molecules with mutations in either of these segments could not transduce a growth signal.
• Gene Symbol: Il6st