Rat Lipocalin-2/NGAL ELISA Kit

CAT#: EA100542

Rat Lipocalin-2/NGAL ELISA Kit


USD 500.00

3 Weeks*

Size
    • 1 x 96 wells

Product Images

Other products for "Lcn2"

Specifications

Product Data
Assay Type Sandwich ELISA kit of Quantitative Detection for Rat Lipocalin-2/NGAL
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 78pg/ml-5,000pg/ml
Sample Type Cell culture supernates, serum, plasma(heparin) and urine.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of rat NGAL
Sensitivity <10pg/ml
Reactivities Rat
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- rat Lipocalin-2/NGAL antibody 1
  • Lyophilized recombinant rat Lipocalin-2/NGAL standard 10ng/tubex2
  • Biotinylated anti- rat Lipocalin-2/NGAL antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Lipocalin-2(LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide(LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant rat NGAL, consisting of 177 amino acids with the molecular mass of 21kDa.
Gene Symbol Lcn2

Documents

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.