Rat IL-3 ELISA Kit

CAT#: EA100795

Rat IL-3 ELISA Kit


USD 430.00

3 Weeks*

Size
    • 1 x 96 wells

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Specifications

Product Data
Assay Type Sandwich ELISA kit of Quantitative Detection for Rat IL-3
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 7.8pg/ml-500pg/ml
Sample Type Cell culture supernates and serum.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of rat IL-3
Sensitivity <10pg/ml
Reactivities Rat
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- rat IL-3 antibody 1
  • Lyophilized recombinant rat IL-3 standard 10ng/tubex2
  • Biotinylated anti- rat IL-3 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5.5 The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.
Gene Symbol Il3

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.