Human Follicle-Stimulating Hormone (FSH) ELISA kit

CAT#: EA100841

Human Follicle-Stimulating Hormone (FSH) ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 5-100 mIU/ml
• Sample Type: Serum
• Sample Volume: 50 µl/well
• Specificity: This kit is used for quantitative detection human Follicle-Stimulating Hormone (FSH)
• Sensitivity: 5 mIU/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Microwells coated with Streptavidin: 12x8x1
  • 2. FSH Standard: 6 vials (ready to use): 0.5ml
  • 3. FSH Enzyme Conjugate: 1 bottle (ready to use): 12 ml
  • 4. TMB Substrate: 1 bottle (ready to use): 12ml
  • 5. Stop Solution: 1 bottle (ready to use): 12ml
  • 6. 20X Wash concentrate: 1 bottle: 25ml
• Background: Follicle-Stimulating Hormone (FSH) is a glygoprotein produced by the anterior pituitary gland. Like other glycoproteins, such as LH, TSH, and HCG, FSH consists of subunits designated as alpha and beta. Hormones of this type have alpha subunits that are very similar structurally; therefore the biological and immunological properties of each are dependent on the unique beta subunit. In the female, FSH stimulates follicular growth, prepares ovarian follicles for action by LH and enhances the LH induced release of estrogen. FSH levels are elevated after menopause, castration and in premature ovarian failure. Although there are significant exceptions ovarian failure is indicated when random FSH concentrations exceed 40 mIU/ml. In the male, FSH stimulates seminiferous tubule and testicular growth and is involved in the early stages of spermatogenesis. Oligospermic males usually have elevated FSH levels. Tumors of the testes generally depress serum FSH concentrations, but levels of LH are elevated. High levels of FSH in men may be found in primary testicular failure and Klinefelter syndrome. Elevated concentrations are also present in cases of starvation, renal failure, hyperthyroidism, and cirrhosis.
• Standard Curve:
  
  Representative standard curve for Follicle-Stimulating Hormone (FSH) ELISA