Human Luteinizing hormone (LH) ELISA kit

CAT#: EA100844

Human Luteinizing hormone (LH) ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 3.1-50 mIU/ml
• Sample Type: Serum
• Sample Volume: 50 µl/well
• Specificity: This kit is used for quantitative detection human Luteinizing hormone (LH)
• Sensitivity: 3.1 mIU/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Microwells coated with Streptavidin: 12x8x1
  • 2. LH Standard: 6 vials ( ready to use): 0.5ml
  • 3. LH Enzyme Conjugate: 1 bottle (ready to use): 12 ml
  • 4. TMB Substrate: 1 bottle (ready to use): 12ml
  • 5. Stop Solution: 1 bottle (ready to use): 12ml
  • 6. Wash concentrate 20X: 1 bottle: 25ml
• Background: Luteinizing hormone (LH) is produced in both men and women from the anterior pituitary gland in response to luteinizing hormone-releasing hormone (LH-RH or Gn-RH), that is released by the hypothalamus. LH, also called interstitial cell- stimulating hormone (ICSH) in men, is glycoprotein with a molecular weight of approximately 30,000 Dalton. It is composed of two noncovalently associated dissimilar amino acid chains, alpha and beta. The alpha chain is similar to that found in human thyroid-stimulating hormone (TSH), follicle-stimulating hormone (FSH), and human chorionic gonadotropin (hCG). LH stimulates ovulation and ovarian steroid production in the female. In the male, LH controls Leydig cell secretion of testosterone. LH is elevated in Luteal phase of menstrual cycle, primary hypogonadism, Gonadotropin-secreting pituitary tumors and menopause. LH is deceased in hypothalamic Gn-RH deficiency, pituitary LH deficiency and ectopic steroid production.
• Standard Curve:
  
  Representative standard curve for Luteinizing hormone (LH) ELISA