Human Luteinizing hormone (LH) Ultra Sensitive lumELISA kit

CAT#: EA100845

Human Luteinizing hormone (LH) Ultra Sensitive lumELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 3.1-50 mIU/ml
• Sample Type: Serum
• Sample Volume: 25 µl/well
• Specificity: This kit is used for quantitative detection human Luteinizing hormone (LH) Ultra Sensitive lu
• Sensitivity: 3.1 mIU/mL
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • Microwells coated with Streptavidin: 6x8x2 wells
  • LH Standard: 7 vials (ready to use): 0.2 ml
  • Biotin Conjugate , 20X: 1 Bottle: 0.7 ml
  • Enzyme Conjugate, 20X: 1 Bottle: 0.7 ml
  • Assay Diluent, 1 bottle (ready to use): 12 ml
  • Luminol Substrate, 3X: 1 Bottle: 4 ml
  • Luminol Buffer: 1 Bottle: 8 ml
  • Wash Concentrate, 20X: 1 Bottle: 25 ml
• Background: Luteinizing hormone (LH) is produced in both men and women from the anterior pituitary gland in response to luteinizing hormone-releasing hormone (LH-RH or Gn-RH), that is released by the hypothalamus. LH, also called interstitial cell- stimulating hormone (ICSH) in men, is glycoprotein with a molecular weight of approximately 30,000 Dalton. It is composed of two noncovalently associated dissimilar amino acid chains, alpha and beta. The alpha chain is similar to that found in human thyroid-stimulating hormone (TSH), follicle-stimulating hormone (FSH), and human chorionic gonadotropin (hCG). LH stimulates ovulation and ovarian steroid production in the female. In the male, LH controls Leydig cell secretion of testosterone. LH is elevated in Luteal phase of menstrual cycle, primary hypogonadism, gonadotropin-secreting pituitary tumors and menopause. LH is deceased in hypothalamic Gn-RH deficiency, pituitary LH deficiency and ectopic steroid production. In children, abnormalities in concentration of LH can be aid in the diagnosis of pituitary disorders, and may be indicative of problems in the reproductive system of both genders, infertility problems, early and delay puberty.