Adrenocorticotropic hormone (ACTH) ELISA (Mouse/Rat) kit

CAT#: EA100849

Adrenocorticotropic hormone (ACTH) ELISA (Mouse/Rat) kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Sample Type: Serum
• Specificity: This kit is used for quantitative detection Adrenocorticotropic hormone (ACTH) (Mouse/Rat)
• Reactivities: Mouse, Rat
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • Microwells coated with Streptavidin: 6x2x8
  • ACTH Standard Zero: 1 bottle, Ready to use: 4 mL
  • ACTH Standards: 5 bottles (Lyophilized): 2 mL
  • Controls 1 & 2 (CTRL) (2 Vials): 2 mL
  • Biotinylated ACTH Antibody (Reagent 1): 2.7 mL
  • Enzyme labeled ACTH Antibody (Reagent 2): 2.7 mL
  • TMB Substrate (Reagent B): 15 ml
  • Stop Solution: 20 ml
  • Wash Concentrate (Reagent A): 30mL
• Background: Adrenocorticotropic Hormone (ACTH) is a 39-amino acid peptide hormone (MW = 4500) secreted mainly by the anterior pituitary gland. Various types of stress or pain perceived in higher levels of the brain modulate secretion of the hypothalamic neurosecretory hormone, corticotropin releasing hormone (CRH). CRH stimulates pituitary ACTH secretion. The second peptide that modulates ACTH secretion is vasopressin (AVP). AVP secretion is also stimulated by stress and acts synergistically with CRH to increase ACTH secretion in the pituitary portal circulation.