Human Cortisol ELISA kit

CAT#: EA100853

Human Cortisol ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Streptavidin based ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 20-400 ng/ml
• Sample Type: Serum
• Sample Volume: 25 µl/well
• Specificity: This kit is used for quantitative detection human Cortisol
• Sensitivity: 20 ng/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Microwells coated with Cortisol MAb: 12x8x1
  • 2. Cortisol Standard: 7 vials ( ready to use): 0.5 ml
  • 3. Biotin Reagent: 1 bottle
  • 4. Enzyme Conjugate (20X): 1.2 ml
  • 5. TMB Substrate: 1 bottle (ready to use): 12 ml
  • 6. Stop Solution: 1 bottle (ready to use): 12 ml
  • 7. 20X Wash concentrate: 1 bottle: 25 ml
  • 8. Assay Diluent: 24 ml
• Background: Cortisol (hydrocortisone, compound F) is the most potent glucocorticoid synthesized from cholesterol. Cortisol is found in the blood either as free Cortisol, or bound to corticosteroid-binding globulin (CBG). Cortisol production has an ACTH- dependent circadian rhythm with peak levels in the early morning and a nadir at night. The factors controlling this circadian rhythm are not completely defined. Serum levels are highest in the early morning and decrease throughout the day. In the metabolic aspect, Cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization. Immunologically, Cortisol functions as an important anti inflammatory, and plays a role in hypersensitivity, immunosuppression, and disease resistance. It has also been shown that plasma Cortisol levels elevate in response to stress. Abnormal Cortisol levels are seen with a variety of different conditions: with adrenal tumors, prostate cancer, depression, and schizophrenia. Elevated Cortisol levels and lack of diurnal variation have been identified in patients with Cushing's disease.