Human Cortisol ELISA (Saliva) kit

CAT#: EA100855

Human Cortisol ELISA (Saliva) kit

Datasheet
SDS

USD 285.00

In Stock*

Size
    • 1 x 96 wells

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Specifications

Product Data
Format 8x12 divisible strips
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hours
Signal Colorimetric
Curve Range 1-80 ng/ml
Sample Type Saliva
Sample Volume 40 µl/well
Specificity This kit is used for quantitative detection human Cortisol (Saliva)
Sensitivity 1 ng/ml
Reactivities Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 1. Microwell coated with Cortisol MAb: 12x8x1
  • 2. Cortisol Standard set: 7 vials ( ready to use): 0.25 ml
  • 3. Enzyme Conjugate (20X): 1.2 ml
  • 4. Wash concentrate (20X): 1 bottle: 25 ml
  • 5. Assay Diluent: 24 ml
  • 6. TMB Substrate: 1 bottle (ready to use): 12 ml
  • 7. Stop Solution: 1 bottle (ready to use): 12 ml
Background Cortisol (hydrocortisone, compound F) is the most potent glucocorticoid synthesized from cholesterol. Cortisol is found in the blood either as free Cortisol, or bound to corticosteroid-binding globulin (CBG). Cortisol production has an ACTH- dependent circadian rhythm with peak levels in the early morning and a nadir at night. The factors controlling this circadian rhythm are not completely defined. saliva levels are highest in the early morning and decrease throughout the day. In the metabolic aspect, Cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization. Immunologically, Cortisol functions as an important anti inflammatory, and plays a role in hypersensitivity, immunosuppression, and disease resistance. It has also been shown that plasma Cortisol levels elevate in response to stress. Abnormal Cortisol levels are seen with a variety of different conditions: with adrenal tumors, prostate cancer, depression, and schizophrenia. Elevated Cortisol levels and lack of diurnal variation have been identified in patients with Cushing's disease

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.