Human Insulin ELISA kit

CAT#: EA100870

Human Insulin ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 6.25-50 µIU/ml
• Sample Type: Serum
• Sample Volume: 25µl/well
• Specificity: This kit is used for quantitative detection human Insulin
• Sensitivity: 6.25 µIU/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Microwell coated with Insulin MAb: 12x8x1
  • 2. Insulin Standards: 6 vials ( ready to use): 0.5ml
  • 3. Insulin Enzyme Conjugate: 1 vial: 0.7ml
  • 4. Assay Diluent: 1 bottle (ready to use): 12ml
  • 5. TMB Substrate: 1 bottle (ready to use): 12ml
  • 6. Stop Solution: 1 bottle (ready to use): 12ml
  • 7. 20X Wash concentrate: 1 bottle: 25ml
• Background: Insulin is the principal hormone responsible for the control of glucose metabolism. It is synthesized in the β- cells of the islets of Langerhans as the precursor, proinsulin, which is processed to form C-peptide and insulin. Both are secreted in equimolar amounts into the portal circulation. The mature insulin molecule comprises two polypeptide chains, the A chain and B chain (21 and 30 amino acids respectively). The two chains are linked together by two inter-chain disulphide bridges. There is also an intra-chain disulphide bridge in the A chain. Insulin concentrations are severely reduced in insulin-dependent diabetes mellitus (IDDM) and some other conditions such as hypopituitarism. Insulin levels are raised in non-insulin- dependent diabetes mellitus (NIDDM), obesity, insulinoma and some endocrine dysfunctions such as Cushing’s syndrome and acromegaly.