Human Pro-Insulin ELISA kit

CAT#: EA100873

Human Pro-Insulin ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 2.6-66 pmol/L
• Sample Type: Serum
• Sample Volume: 100µl/well
• Specificity: This kit is used for quantitative detection human Pro-Insulin
• Sensitivity: 2.6 pmol/L
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Microwells coated with anti Pro-insulin Antibody: 12x8x1
  • 2. Pro-Insulin Standards: 6 vials ( ready to use): 1ml
  • 3. Pro-Insulin Enzyme Conjugate11X: 1 vial: 1.2 ml
  • 4. TMB Substrate: 1 bottle (ready to use): 14mL
  • 5. Stop Solution: 1 bottle (ready to use): 14 ml
  • 6. Wash concentrate 40X: 1 bottle: 30 ml
  • 7. Sample Diluent : 1 vial (ready to use): 2 mL
  • 8. Conjugate Diluent: 1 bottle (ready to use): 12 mL
  • 9. control (low & high): 2 ml
  • 10. Assay Buffer: 1 bottle (ready to use): 12 mL
• Background: Proinsulin is a 9390 MW polypeptide of 86 amino acids that is synthesized in the β cells of the pancreas and is the precursor molecule for insulin. Most proinsulin is converted to insulin and C-Peptide, which are secreted in equimolar amounts into the blood. About 15 % is not converted and is released as proinsulin. The biological activity of proinsulin is only about 10% of Insulin, but the half life of proinsulin is three times as long as insulin. The level of proinsulin in serum can be a reflection of β cell status. Both IDDM and NIDDM are characterized by dysfunction of the pancreatic β cells. Elevated proinsulin levels have been noted at the onset of IDDM and in healthy siblings of IDDM patients. Proinsulin levels may also be increased in patients with established NIDDM. Increased levels of circulating proinsulin are found in older patients, pregnant or obese diabetics, and patients with insulinomas, functional hypoglycemia and hyperinsulinemia, a rare syndrome. Because the structure of proinsulin is similar to insulin, proinsulin may be detected as immunoreactive insulin in the insulin assay. Immunoreactive insulin levels are generally determined in conventional RIA's, which overestimate the insulin level because the methods use antibodies which cross-react with proinsulin. By calculating the molar ration of proinsulin to true insulin (P/I), a better assessment of β cell function can be made.