Human IgE ELISA kit

CAT#: EA100874

Human IgE ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Sample Type: Serum
• Specificity: This kit is used for quantitative detection human IgE
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Microwells coated with IgE MAb: 12x8x1
  • 2. IgE Standard: 6 vials ( ready to use): 0.5ml
  • 3. IgE Enzyme Conjugate: 1 bottle (ready to use): 12 ml
  • 4. Assay Diluent: 1 bottle (ready to use): 12 ml
  • 5. TMB Substrate: 1 bottle (ready to use): 12ml
  • 6. Stop Solution: 1 bottle (ready to use): 12ml
  • 7. 20X Wash concentrate: 1 bottle: 25ml
• Background: IgE constitutes a fraction of the total antibody in serum 50-300 ng/ml (compared to 10 mg/ml for IgG) and together with its Fc receptor is important in primary immune responses. The immunogenetic mechanisms underlying IgE responsiveness seen in the atopic diseases can be divided into antigen-specific and non- antigen-specific responses. IgE antibodies to common antigens are reported in the serum of 13% of normal blood donors. Autoantibodies to the IgE Fc-epsilon-RII (high affinity receptors) reported in the sera of patients with chronic urticaria, can induce histamine release from mast cells. Patients with atopic allergic diseases such as atopic asthma, atopic dermatitis, and hay fever have been shown to exhibit increased total immunoglobulin E (IgE) levels in blood. IgE is also known as the reagenic antibody. In general, elevated levels of IgE indicate an increased probability of an IgE-mediated hypersensitivity, responsible for allergic reactions. Parasitic infestations such as hookworm, and certain clinical disorders including aspergillosis, have also been demonstrated to cause high levels of IgE. Decreased levels of IgE are found in cases of hypogammaglobulinemia, autoimmune diseases, ulcerative colitis, hepatitis, cancer, and malaria. Cord blood or serum IgE levels may have prognostic value in assessing the risk of future allergic conditions in children. Certain groups of white blood cells, including basophils and tissue mast cells, have membrane receptors for the IgE molecule.