Human 25(OH) Vitamin D ELISA kit

CAT#: EA100877

Human 25(OH) Vitamin D ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 2.5-150 ng/ml
• Sample Type: Serum
• Sample Volume: 10 µl/well
• Specificity: This kit is used for quantitative detection human 25(OH) Vitamin D
• Sensitivity: 2.5 ng/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Microwell plate coated with anti-Vitamin D: 12x8x1
  • 2. Vitamin D Standard Set: 7 vials (ready to use): 0.5 ml
  • 3. Vitamin D Control Set: 2 vials (ready to use): 0.5 ml
  • 4. Biotinylated 25(OH)D Reagent: 1 Vial (51X): 0.55 ml
  • 5. Assay Diluent, 1 bottle: 24 ml
  • 6. Streptavidin-HRP, 1 bottle (ready to use): 23 mL
  • 7. Stop Solution, 1 bottle (ready to use): 12 mL
  • 8. TMB Substrate, 2 bottles (ready to use): 2 x 12 ml
  • 9. Microplate sealing film: 2
  • 10. Wash Concentrate 20X, 1 bottle: 25 ml
• Background: Vitamin D is a steroid hormone involved in the active intestinal absorption of calcium and in the regulation of its homeostasis. Vitamin D has two isomers: Vitamin D2 and Vitamin D3. Vitamin D2 is obtained from dairy products whereas Vitamin D3 is produced in the skin after exposure to ultraviolet light. In the liver, Vitamin D is hydroxylated at its carbon 25 to form 25-OH Vitamin D. This metabolite is the predominant circulating form of Vitamin D and is considered to be an accurate indicator of the general Vitamin D status of an individual. Vitamin D deficiency has been linked to many diseases including osteoporosis, rickets, osteomalacia, cancers, and cardiovascular diseases. Both dietary supplements of Vitamin D that are currently available in the market (Vitamin D2 and Vitamin D3) are converted to 25-OH Vitamin D in the liver. The sum of the concentrations of 25-OH Vitamin D2 and 25-OH Vitamin D3, in serum or plasma, is referred to as “Total 25-OH Vitamin D”. Accurate monitoring of total 25-OH Vitamin D level is critical in clinical settings. Vitamin D deficient patients who are prescribed a daily Vitamin D supplement should regularly monitor their serum or plasma Vitamin D levels in order to reach an optimal level and prevent their 25-OH Vitamin D concentrations from reaching excessive levels that are considered toxic1-5
• Standard Curve:
  
  Representative standard curve for 25(OH) Vitamin D ELISA