Human PTH ELISA kit

CAT#: EA100878

Human PTH ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 0-900 pg/ml
• Sample Type: Serum
• Sample Volume: 25 µl/well
• Specificity: This kit is used for quantitative detection human PTH
• Sensitivity: 0.49 pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • Microwells coated with Sterptavidin: 12x8x1
  • PTH Standard 6: 1 Vial (lyophilized): 0.75 ml
  • PTH Controls: 2 Vials (lyophilized): 0.75 ml
  • Anti-PTH-Biotin Reagent: 1 Bottle (Ready to use): 7 ml
  • Anti-PTH-HRP Enzyme Conjugate: 1 Bottle (Ready to use): 7 ml
  • Assay Diluent: 1 Bottle (ready to use): 5 ml
  • TMB Substrate: 1 Bottle (Ready to use): 12 ml
  • Stop Solution: 1 Bottle (Ready to use): 12 ml
  • 20X Wash Solution: 1 Bottle: 25 ml
• Background: PTH (Parathyroid hormone) is biosynthesized in the parathyroid gland as a pre-proparathyroid hormone, a large molecular precursor consisting 115 amino acids. In healthy individuals, regulation of parathyroid hormone secretion normally occurs via a negative feedback action of serum calcium on the parathyroid glands. Intact PTH is biologically active and clears very rapidly from the circulation with a half-life of less than four minutes. Intact PTH assays are important for the differentiation of primary hyperparathyroidism from other (non-parathyroid-mediated) forms of hypercalcemia, such as malignancy, sarcodosis and thyrotoxicosis. The measurement of parathyroid hormone is the most specific way of making the Diagnosis of primay hyperparathyroidism. In the presence of hypercalcemia, an elevated level of parathyroid hormone virtually establishes the diagnosis. In over 90% of patents with primary hyperparathyroidism, the parathyroid hormone will be elevated. The most common other cause of hypercalcemia, namely hypercalcemia of malignancy, is associated with suppressed levels parathyroid hormone or PTH levels within the normal range. PTH values are typically undetectable in hypocalcemia due to total hypoparathyroidism, but are found within the normal range in hypocalcemia due to partial loss or inhibition of parathyroid function.