Morphine (Canine) ELISA kit

CAT#: EA100909

Morphine (Canine) ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Sample Type: Serum
• Sample Volume: 20 µl/well
• Specificity: This kit is used for quantitative detection Morphine (Canine)
• Reactivities: Canine
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Microwell with polyclonal anti-morphine: 12x8x1
  • 2. Morphine-Conjugate: 12.5 ml
  • 3. Morphine Positive Ref. Std: 1 ml
  • 4. Neg Std: 1 ml
  • 5. TMB Substrate: 14ml
  • 6. Stop Reagent: 12.5ml
• Background: The Morphine Specific Direct ELISA Kit is based upon the competitive binding to antibody of enzyme labeled antigen and unlabeled antigen, in proportion to their concentration in the reaction mixture. A 20 µl aliquot of a diluted unknown specimen is incubated with a 100 µl dilution of enzyme (Horseradish peroxidase) labeled morphine derivative in micro- plate wells, coated with fixed amounts of oriented high affinity purified polyclonal antibody. The wells are washed thoroughly and a chromogenic substrate added. The color produced is stopped using a dilute acid stop solution and the wells read at 450 nm. The intensity of the color developed is inversely proportional to the concentration of drug in the sample. The technique is sensitive to 1 ng/ml. The Morphine Specific Direct ELISA Kit avoids extraction of urine sample for measurement. It employs a Morphine Specific directed antiserum. Due to the proprietary method of orienting the antibody on the polystyrene micro-plate much higher sensitivity is achieved compared to passive adsorption. This allows an extremely small sample size reducing matrix effects and interference with binding proteins(s) or other macromolecules.